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BN40893R-100ul
100ul
￥2470.00
交叉反應：nan(predicted:Mouse,Rat,Dog) 推薦應用：WB,ELISA
產(chǎn)品描述
| 英文名稱 | Phospho-Mre11 (Ser676) |
| 中文名稱 | 磷酸化DNA損傷關(guān)鍵蛋白Mre11抗體 |
| 別 名 | Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 meiotic recombination 11 homolog A; MRE11a; MRE11b; AT like disease; Ataxia telangiectasia disorder like; Ataxia-telangiectasia disorder-like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11. HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; meiotic recombination 11 homolog A (S. cerevisiae); meiotic recombination 11 homolog A; MmMRE11A.MRE11_RAT |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Mouse, Rat, Dog, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 80kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676:SQ(p-S)Q |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation Subunit: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Subcellular Location: Nucleus. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. Similarity: Belongs to the MRE11/RAD32 family. SWISS: P49959 Gene ID: 64046 Database links: Entrez Gene: 4361 Human Entrez Gene: 17535 Mouse Omim: 600814 Human SwissProt: P49959 Human SwissProt: Q61216 Mouse Unigene: 192649 Human Unigene: 149071 Mouse Unigene: 209040 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 在細胞中，有多種蛋白參與DNA損傷應答，DNA損傷是可引起癌變的細胞變化，這些蛋白在細胞發(fā)生損傷后會啟動細胞修復過程，幫助受損的細胞恢復正常。 正常情況下，細胞會經(jīng)歷生長、分化和自然死亡的歷程。當細胞受到損傷時，如;輻射損傷或是毒物刺激，一種多蛋白復合物參與的步驟將被啟動，進行細胞修復工作并激活其他的生物過程。在這過程中，存在一種MRN復合物，由Mre11，Rad50和NBS1蛋白組成，MRN探測DNA損傷（DNA雙鏈是否斷裂）的情況。復合物在探測到DNA損傷信號后將把這個信息傳遞給一種酶，ATM（ataxia-telangiectasia mutated）檢測激酶（checkpoint kinase）。ATM激酶能對DNA雙鏈斷裂產(chǎn)生應答反應，它具有降低細胞增殖的能力，給細胞修復騰出時間。因此ATM一旦發(fā)生變異，功能失效可能導致免疫缺陷甚至是癌變。 研究者認為，Mre11不僅是DNA損傷的感受器，更是修復DNA的啟動因子，還能修飾受損的DNA分子 |
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